Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole sheila a. Initial characterization of corynespora cassiicola affecting florida tomatoes pdf. The journal publishes papers that describe translational and applied research focusing on practical aspects of disease diagnosis, development, and management in agricultural and horticultural crops. Increasing sporulation of corynespora cassiicola springerlink. Free fulltext pdf articles from hundreds of disciplines, all in one place detection of corynespora cassiicola in hevea rubber tree from china pdf paperity toggle navigation. Pathogenicity of corynespora cassiicola on different hosts in. The genus mandevilla includes many lush, showy, flowering tropical plants. Pdf target spot, caused by the fungus corynespora cassiicola, occurs endemically in amazonas state, leading to reduced production in several. Pathogenicity of corynespora cassiicola on different hosts. The organism was isolated and identified as corynespora cassiicola according to its morphological characteristics and. Corynespora cassiicola isolates resistant to boscalid were detected in 17 greenhouses which consisted of eight with a history of two or three applications of boscalid per crop, and nine with only one application.
Few studies referred to the formation of chlamydospores by c. A small secreted glycoprotein named cassiicolin was previously described as an important effector of c. The necrotrophic fungus corynespora cassiicola berk. The fungus corynespora cassiicola is primarily found in the tropics. Species in the genus induce corynespora leaf spot in bleeding hearts clerodendrum thomsoniae. These metrics are regularly updated to reflect usage leading up to the last few days. Corynespora cassiicola is a common plant pathogen that causes leaf spot disease in a broad range of crop, and it heavily affect rubber trees in malaysia hsueh, 2011. Occurrence of corynespora cassiicola isolates resistant to. Corynespora cassiicola is causing numerous emerging diseases, including target spot of cotton a disease is emerging if increase in host range, geographic range, or incidence is observed, or new strains evolve. Jsv provided expertise for the manual tuning of clustering parameters. The target genes of these mirnas were related to secondary metabolism. Due to its high sensitivity, specificity, and reliability, nested pcr is useful for the screening and certification of young c. Plant disease is the leading international journal for rapid reporting of research on new, emerging, and established plant diseases. Cucumber target spot, cucumber leaf spot, corynespora blight.
It has been reported in more than 70 host plants including economically important crops such as rubber tree, tomato, cucumber, cotton, soybean, tobacco, cocoa, cowpea,1. In the last few decades it has become one of the most destructive foliar and fruit diseases of tomato in the state mackenzie et al. Structural analysis of cassiicolin, a hostselective. No known resistance to target spot is currently available in commercial cultivars. No resistance was observed on 10 varie ties of cucumbers inoculated. Hostspecific toxin production by the tomato target leaf. Initial characterization of corynespora cassiicola affecting florida tomatoes. Pdf the fungus corynespora cassiicola is an important pathogen that causes necrotic lesions in several plant species. Diagnostic features of corynespora cassiicola and its. It was first reported on tomato lycopersicon ecsulentum mill. View the article pdf and any associated supplements and figures for a. Subcutaneous infection caused by corynespora cassiicola, a. Functional identification of corynespora cassiicola. These climatic conditions would limit the ability of the pathogen to.
However, the underlying factors that contribute to the opportunistic cross. The fungus can infect the leaves, stems, and flowers of azaleas and. Extensive superficial necrotic lesions developed on seedlings from inoculated seeds planted in the field in midmay, but few symptoms. Diversity of the cassiicolin gene in corynespora cassiicola. A necrotic subcutaneous lesions over the dorsal aspects of both hands with purulent discharge in a patient with chronic subcutaneous infection caused by corynespora cassiicola. Previous studies revealed that populations from the three hosts are genetically distinct and host specialized. A new foliar disease, corynespora leaf spot, has been. Gao wei 1,2, li baoju 2, wang wanli 1, hao yongjuan 1, shi yanxia 2. Frequency distribution of ec 50 values gml for thiophanatemethyl from 40 greenhousecollected isolates of corynespora cassiicola from african violet in 2007 displayed with a maximum xaxis value of 1.
Functional identification of corynespora cassiicolaresponsive. Corynespora cassiicola on papaya causing small angular spots. The same test was performed in the presence of 6 m guhcl, in order to unfold the protein, thus facilitating the. Genetic determinism of sensitivity to corynespora cassiicola.
In the rubber tree, this fungus causes the corynespora leaf fall clf disease, which increasingly affects natural rubber production in asia and africa. All necessary for pest control diseases of mandevilla. The planar structures were determined by extensive nmr spectroscopic data. The asexual stage of the fungus was reisolated from leaf spots on inoculated leaves.
Transcriptome and mirna analyses of the response to. The disease occurs on a wide range of host plants in both tropical and subtropical countries. A total of 651 isolates of cucumber corynespora leaf spot fungus corynespora cassiicola collected from cucumber in japan, either with 438 isolates or without 2 isolates a prior history of boscalid use, were tested for their sensitivity to boscalid by using a mycelial growth inhibition method on yba agar medium. Pdf purification and characterization of cassiicolin. Genomewide analysis of corynespora cassiicola leaf fall disease. Molecules free fulltext naphthalenones and depsidones. A leaf spot of cucumber, prevalent in collier county, florida has been shown to be caused by corynespora cassiicola. This fungus is present in some hevea rubber plantations and nurseries in hainan and yunnan provinces of south china. A total of 651 isolates of cucumber corynespora leaf spot fungus corynespora cassiicola collected from cucumber in japan, either with 438 isolates or without 2 isolates a prior history of bos. An indirect phenotyping method was developed in order to estimate the susceptibility of rubber tree clonal varieties to corynespora leaf fall clf disease caused by the ascomycete corynespora cassiicola. Saprophytism and parasitism habits of plants are common survival strategies for this pathogen. Corynespora cassiicola was pathogenic on the roots and hypocotyls of soybean seedlings grown in infested soil at ottawa.
Mycelial growth of mr and vhr isolates on boscalid. Pathogenic and genetic variation among isolates of. Initial characterization of corynespora cassiicola and. Corynespora cassiicola corycaoverview eppo global database. In addition, the pathogen could be detected from artificially infected hevea rubber trees 3 days after inoculation. Corynespora cassiicola factors favoring the disease.
These problems usually occur during warm, summer months. The tested exudates were either crude culture filtrates from diverse c. Keep plots free from weeds, as some may be hosts of the fungus. The disease appeared early enough in the fall season of 1966 to carry out field spray tests to determine what fungicides would best control it. A new foliar disease, corynespora leaf spot, has been detected in arkansas cotton travis faske extension plant pathologist corynespora leaf spot has been detected in several commercial cotton fields on a few varieties fm 1944, st 5458, and phy 499 in craighead, mississippi, poinsett, and woodruff counties. Genetic determinism of sensitivity to corynespora cassiicola exudates in rubber tree hevea brasiliensis. Clonality and geographic structure of hostspecialized. The humidity may be used as a surrogate for leaf wetness, as it is an essential component of an environment and has.
Corynespora cassiicola infection is common in plants, but the human corynespora cassiicola infection in our report is rare according to the literature. Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole article pdf available in tropical plant pathology 385. Wei, is a common disease in almost all tomato growing regions of the world ellis and holliday, 1971. Article views are the countercompliant sum of full text article downloads since november 2008 both pdf and html across all institutions and individuals. Among them, 12, 34, 56, and 78 were pairs of epimers. Corynespora cassiicola is causing numerous emerging diseases, including target spot of cotton a disease is emerging if increase in host range, geographic range, or.
The absolute configurations of 110 were assigned by the modified moshers method and tddft ecd calculations. Genome sequences and snp analyses of corynespora cassiicola. Corynespora leaf spot caused by corynespora cassiicola is one of the. Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole da manchaalvo, corynespora cassiicola, na safra 201 112. During propagation and pruning, cutting utensils should be sterilized between each cut. Corynespora cassiicola is an important plant pathogenic ascomycete causing the damaging corynespora leaf fall clf disease in rubber tree hevea brasiliensis. Pacific pests and pathogens fact sheets cucumber target spot 189 common name cucumber target spot, cucumber leaf spot, corynespora blight scientific name corynespora cassiicola distribution worldwide. Target spot, corynespora cassiicola, develops rapidly on tomato when conditions are suitable for the fungus. If pots and trays from contaminated plants must be reused, they should be. Pdf pathogenicity of corynespora cassiicola on different hosts in. Pdf resumo many diseases are affecting tomato fruits in the field and. Wei was greatly enhanced when the cultures were first scraped after 3 days growth and then grown for an additional 3 days in continuous light. The risk corynespora cassiicola would pose to california is evaluated below.
Detection of corynespora cassiicola in hevea rubber tree. A total of 651 isolates of cucumber corynespora leaf spot fungus corynespora cassiicola collected from cucumber in japan, either with 438 isolates or without 2 isolates a prior histo ry of boscalid use, were tested for their sensitivity to boscalid by using a mycelial growth inhibition method on. Pdf corynespora leaf spot of cucumber semantic scholar. Differentiation of corynespora cassiicola and cercospora sp. No absorption was measured at 412 nm, indicating no release of tnb in solution due to the reaction of dtnb with a free sh group. Structural analysis of cassiicolin, a hostselective protein.
Though generally hardy tropicals, the plants can suffer from a variety of pests and problems, such as spider mites, corynespora cassiicola fungus and leaf spot. Symptoms on leaves included fishbone necrosis and spots. Detection of corynespora cassiicola in hevea rubber tree from. We report a case of subcutaneous phaeohyphomycosis caused by a plant pathogen in a patient with acute heart failure. The causal organism was isolated in pure culture and identified ascorynespora cassiicola berk. This fungal infection is one of the more common in the southern states and more humid areas of the united states. Two new naphthalenones, corynenones a and b 1 and 2, and one new depsidone, corynesidone e 3, together with one known depsidone, corynesidone a 4 and two known diphenyl ethers, corynethers a 5 and b 6, were isolated from the spongederived fungus corynespora cassiicola xs20090i7. Host plants carriers of corynespora can be wide in variety. A new boll rot disease of cotton gossypium hirsutum l.
Nested pcr assay for detection of corynespora leaf fall. Corynespora cassiicola has been found for the first time in china on hevea rubber tree hevea brasiliensis in south china during a survey in 2006. Pdf corynespora brown spot of papaya corynespora cassiicola. Strobilurins and related compounds are prone to the development of resistance and should be rotated with other chemistries. Eco ri, msp i and rsa i endonucleases identified all isolates as corynespora cassiicola. It is a member of the mitosporic ascomycota, a heterogeneous group of ascomycotic fungi whose common characteristic is the absence of a sexual state. Pdf sensitivity of corynespora cassiicola from soybean. Five genetic groups were identified using rapdpcr profiles generated by eight random primers. Corynespora cassiicola is an important pathogen because its responsible for a considerable loss of crop production and yield. Target spot, corynespora cassiicola, defoliating tomato plants.
Pacific pests and pathogens fact sheets tomato target spot 163 common name. Their structures including absolute configurations were determined by spectroscopic data and electronic. Target spot, caused by the fungus corynespora cassiicola, occurs endemically in amazonas state. Wei is a cosmopolitan plant pathogen, especially abundant in the tropics. The isolation of um 591 from a patients contact lens indicates the pathogenic potential of this dematiaceous fungus in human. Detection of corynespora cassiicola in hevea rubber tree from china. Pdf on sep 10, 2012, jose ricardo liberato and others published corynespora brown spot of papaya corynespora cassiicola find, read and cite all the. Genetic variation of 42 isolates of corynespora cassiicola, a destructive fungal pathogen of many economically important crop plants including rubber, was investigated using rapdpcr analysis. Although variation in aggressiveness to cotton and tomato were observed, no genetic diversity. Structural analysis of cassiicolin, a hostselective protein toxin from corynespora cassiicola. Distribution and molecular characterization of corynespora. Detection of corynespora cassiicola in soil with realtime quantitative pcr. It affects many types of plants, including flowering ornamentals and vegetable crops. Corynespora cassiicola is a species of fungus well known as a plant pathogen.
Purification and characterization of cassiicolin, the toxin produced by corynespora cassiicola, causal agent of the leaf fall disease of rubber tree. Corynespora cassiicola is a destructive plantpathogenic fungus causing widespread target spot epidemics, including outbreaks on cotton, soybean, and tomato in the southeastern united states. Moreover, resistant isolates were also obtained from two greenhouses tsukuba. Detection of corynespora cassiicola in soil with realtime. Chromone derivatives from a spongederived strain of the fungus corynespora cassiicola. The fungus corynespora cassiicola is an important pathogen that causes necrotic lesions in several plant species.
Genomic insight into pathogenicity of dematiaceous fungus. Free fulltext pdf articles from hundreds of disciplines, all in one place. Mostly known as a necrotrophic plant pathogen, it has also been associated with rare cases of human infection. Chromone derivatives from a spongederived strain of the. This is an open access article, free of all, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used. Genetic variation in corynespora cassiicola cambridge core. Host specialization and phylogenetic diversity of corynespora. Pdf symptomatology, hosts, and pathogenicity of corynespora. Closeup, angular leaf spots, coynespora cassiicola, on cucumber.
This disease occurs on both green and variegated forms of f. Growth sensitivity of corynespora cassiicola to thiophanate. Corynespora cassiicola is an ascomycetes fungus with a broad host range and diverse life styles. In other words, the leaf wetness is likely a major environmental factor driving the disease for this fungal pathogen. Twelve new chromone derivatives, corynechromones al 112, were isolated from the spongederived fungus corynespora cassiicola xs200900i7. Frontiers genomewide analysis of corynespora cassiicola. Pdf sensitivity of corynespora cassiicola from soybean to. Diagnostic features of corynespora cassiicola and its associated diseases identifying c. A total of 618 isolates of corynespora leaf spot fungus corynespora cassiicola collected from 24 commercial cucumber greenhouses in 12. First record of corynespora leaf fall disease of hevea. Cookies are small text files that contain a string of characters and uniquely identifies a browser.
Corynespora cassiicola, which causes corynespora leaf fall disease, is one of the most destructive and economically important fungal pathogens of hevea rubber trees in asian and african countries. Free fulltext pdf articles from hundreds of disciplines, all in one place genetic determinism of sensitivity to corynespora cassiicola exudates in rubber tree hevea brasiliensis pdf paperity toggle navigation. Target spot of tomato, caused by corynespora cassiicola, was first reported in florida in 1972. Sequence data from the ribosomal its operon placed the isolates within the corynespora cassiicola complex. Corynespora cassiicola exudates in rubber tree hevea. In order to develop a method for discrimination of corynespora cassiicola isolates pathogenic to sweet pepper among japanese isolates, this study analysed pathogenic variations of 64 japanese isolates of c. The isolate, which on cotton is pathogenic only to bolls, is considered to be different from a strain ofc. Target leaf spot tls, which is caused by corynespora cassiicola c. However, no polymorphisms were found at the its sequences. Occurrence of corynespora cassiicola isolates resistant to boscalid. A new foliar disease, corynespora leaf spot, has been detected in arkansas cotton travis faske extension plant pathologist corynespora leaf spot has been detected in several commercial cotton fields on a few varieties fm 1944, st 5458, and phy 499 in. Two primer pairs showed specificity to corynespora cassiicola and one primer pair showed specificity to cercospora sp.
Initial characterization of corynespora cassiicola and alternaria spp. Sensitivity of corynespora cassiicola from soybean to. Cassiicola known elsewhere causing leaf spot of cotton. Morphological and molecular characterization of isolates.
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